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Final Report

COST WG D25/0002/02

  • New microbial strains and enzymes were discovered and largely made available to WG partners. These new biocatalysts comprise the whole spectrum of enzymes involved in hydrolytic nitrile degradation pathways (nitrile hydratase, amidase, nitrilase, aldoxime dehydratase; see Scheme 5) and secondary amide hydrolase.
  • Different novel approaches proved to be viable for immobilization of nitrile- and amideconverting enzymes (retention of nitrilase in an ultrafiltration membrane bioreactor, crosslinked enzyme aggregate technology, adsorption to hydrophobic supports like Butyl Sepharose) and to their stabilization (via co-aggregation with polyethyleneimine). The first two methods have been applied during the STSMs (O. Kaplan to Univ. L´Aquila, D. Kubá_ to TU Delft).
  • A broad spectrum of new substrates including chiral compounds (e.g., substituted cyclopentane- and cyclohexanecar­bonitriles, 3-hydroxynitriles, 2-oxonitriles, 2-halonitriles and 2-amino nitriles; hexosamine derivatives) bearing different protecting groups have been prepared as starting materials for the synthesis of useful synthons and their transformation accomplished in a chemo-, regio- or stereoselective way using the above enzymes.
  • The above achievements have been largely published (17 articles, 2 book chapters publishhed or accepted; further three joint articles are in preparation – to be submitted by the end of 2007). In addition, WG2 presented about 25 joint conference communications. Please see below for a list of joint articles, book chapters and abstracts.
  • The activities of the WG promoted the participation of young microbiologists, biochemists and organic chemists. Throughout WG2, the staffs contained a high ratio of postgraduate students and postdocs. Undergraduate students also participated in the research. The young scientists significantly contributed to the publication output of WG2. The participation in the D25 Action enabled them to present their works at workshops and WG meetings. Particularly, the short-term scientific missions were important for the enhancement of qualification of the young scientists. All the six STSMs were accomplished by PhD students and afforded a valuable output, as documented by the STSM reports. These efforts were also supported by a bilateral project between WG members: Bilateral collaboration between the Lab. of Biotransformation Prague and University Hohenheim has been granted with financial support from DAAD and the Acad. Sci. Czech Rep. for 2006–2007. The topic of this project (“Preparation of chiral synthons from nitriles”) is a continuation of the WG2 programme and enabled other four student exchanges in 2006–2007 (in total duration of about 12 weeks).

Read the (PDF, 2MB) of the COST D25 action.

COST Actions

Institute of Microbiology - Czech Academy of Sciences