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The ways to N-acetylmannosamine structures via mannoside metabolizing enzymes

(, B. Fliedrová, Z. Matějčková, K. Křenek, V. Křen)

Synthesis of N-acetylmannosamine glycosides and its other derivatives remain still a great challenge for carbohydrate chemists. This carbohydrate is gaining recently high importance as , pathology marker in some bacteria and precursor of (modified) sialic acids. Recently, series of chemical synthesis of ManNAc containing compounds were published but these syntheses are very complicated. To our best knowledge, β-N-acetylmannosidases, which could catalyze the cleavages/enzymatic synthesis of β-ManNAc containing structures, have not been described yet. This project is focused on the searching for enzymes with β-N-acetylmannosidase activities and parallel on the construction of a mutant β-mannosidase with altered biocatalytic abilities, aiming at β-N-acetylmannosidase activity.

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Institute of Microbiology - Czech Academy of Sciences