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RNDr. Pavla Bojarová, PhD.

Photo: Pavla Bojarová
Position:
Senior Scientist
Email:
bojarova (at) biomed.cas.cz
Phone:
(+420) 296 442 360
Address:

Laboratory of Biotransformation,
Institute of Microbiology,
Academy of Sciences of Czech Republic

Videňská 1083
Praha 4
142 20

Czech Republic

Alternative Email

pavla (at) bo­jar.ruk.cuni.cz

Education

PhD Thesis

Employment

Since 2010 Lecturer at Department of Biochemistry, Faculty of Science, Charles University in Prague (Biochemistry and Biomedicine of Carbohydrates)
Since 2008 Research fellow in the Laboratory of Biotransformation
2006–2007 Postdoctoral stay at the University of Melbourne, Australia
Since 1999 member of the Laboratory of Biotransformation

Study stays

Year Institute Length
2010 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 2 weeks
2006–7 School of Chemistry, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Australia 13 mths
2006 Directed Enzyme Evolution Course, Inst. Biotechnology, Vilnius 1 week
2005 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 7 weeks
2005 Istituto di Chimica del Riconoscimento Molecolare, CNR, Milan 4 weeks
2003 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 3 mths
2003 Departamento de Quimica organica, Fac.de Quimica, Univ. de Sevilla 4 weeks
2002 Institut für Organische Chemie, Universität Hamburg 4 weeks

Publications & Presentations

Publications

  1. P. Fialová, L. Weignerová, J. Rauvolfová, V. Přikrylová, A. Pišvejcová, R. Ettrich, M. Kuzma, P. Sedmera, V. Křen: Hydrolytic and Transglycosylation Reactions of N-Acyl Modified Substrates Catalysed by -N-Acetylhexosami­nidases. Tetrahedron 2004, 60, 693701.
  2. J. Rauvolfová, L. Weignerová, P. Fialová, V. Přikrylová, M. Kuzma, A. Pišvejcová, V. Křen: -N-Acetylhexosami­nidase Catalysed Synthesis of Non-Reducing Oligosaccharides. J. Mol. Catal. B: Enzymatic 2004, 29, 233239.
  3. P. Fialová, L. Elling, D.-J. Namdjou, R. Ettrich, M. Kuzma, V. Přikrylová, J. Rauvolfová, K. Bezouška, V. Křen: Combined Application of Galactose Oxidase and -N-Acetylhexosami­nidase in the Preparation of Complex Bioactive N-Acetylhexosami­nides. Adv. Synth. Catal. 2005, 347, 9971006.
  4. P. Fialová, A. T. Carmona, I. Robina, R. Ettrich, P. Sedmera, V. Přikrylová, L. Petrásková-Hušáková, V. Křen: Glycosyl Azide – A Novel Substrate for Enzymatic Transglycosyla­tions. Tetrahedron Lett. 2005, 46, 87158718.
  5. A. T. Carmona, P. Fialová, V. Křen, R. Ettrich, L. Martínková, A. J. Moreno-Vargas, C. Gonzales. I. Robina: Cyanodeoxy-Glycosyl Derivatives as Substrates for Enzymatic Reactions. Eur. J. Org. Chem. 2006, 18761885.
  6. P. Bojarová-Fialová, V. Křen: Enzymatic approaches to O-glycoside introduction: Glycosidases. In Comprehensive Glycoscience (J. P. Kamerling, Ed.); Elsevier: Oxford, 2007.
  7. O. Kaplan, V. Vejvoda, O. Plíhal, P. Pompach, D. Kavan, P. Bojarová, K. Bezouška, M. Macková, M. Cantarella, V. Jirků,3V. Křen, L. Martínková: Purification and Characterization of a Nitrilase from Aspergillus niger K10. Appl. Microbiol. Biotechnol., 2006, 73, 567575.
  8. P. Bojarová, L. Petrásková, E. Ferrandi, D. Monti, H. Pelantová, M. Kuzma, P. Simerská, Vladimír Křen: Glycosyl Azides – An Alternative Way to Disaccharides. Adv. Synth. Catal., 2007, 349, 1514–1520.
  9. P. Bojarová, K. Křenek, M. Kuzma, L. Petrásková, K. Bezouška, D.-J. Namdjou, L. Elling, V. Křen: N-Acetylhexosamine triad in one molecule: Enzymatic introduction of 2-acetamido-2-deoxy--D-galactopyrano­syluronic acid residue into a complex oligosaccharide. J. Mol. Catal. B: Enzymatic, 2008, 50, 69–73.
  10. P. Bojarová, E. Denehy, I. Walker, K. Loft, D. P. De Souza, L. W. L. Woo, B. V. L. Potter, M. J. McConville, S. J. Williams: Direct Evidence for ArO-S Bond Cleavage upon Inactivation of Pseudomonas aeruginosa Arylsulfatase by Aryl Sulfamates. ChemBioChem 2008, 9, 613–623.
  11. P. Bojarová, S. J. Williams: Sulfotransferases, sulfatases and formylglycine-generating enzymes: a sulfation fascination. Curr. Opin. Chem. Biol. 2008, 12, 1–9.
  12. P. Bojarová, S. J. Williams: Aryl sulfamates are broad spectrum inactivators of sulfatases: Effects on sulfatases from various sources. Bioorg. Med. Chem. Lett. 2009, 19, 477–480.
  13. K. J. Loft, P. Bojarová, K. Slámová, V. K en, S. J. Williams: Synthesis of Sulfated Glucosaminides for Profiling Substrate Specificities of Sulfatases and Fungal -N-Acetylhexosami­nidases. ChemBioChem 2009, 10, 565–576.
  14. P. Bojarová, V. Křen: Glycosidases: a key to tailored carbohydrates. Trends Biotechnol. 2009, 27, 199–209.
  15. P. Bojarová, K. Křenek, K. Wetjen, K. Adamiak, H. Pelantová, K. Bezouška, L. Elling, V. Křen: Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltran­sferase – way to new glycodrugs and materials. Glycobiology 2009, 19, 509 – 517.
  16. L. Weignerová, P. Bojarová, V. Křen: Glycosidases in synthesis. In Carbohydrate Chemistry. Chemical and Biological Approaches, 35, 310–332 (2009).
  17. V. Křen, P. Bojarová: Glycosyl Azides – Novel Substrates for Enzymatic Transglycosyla­tions. In Practical Methods in Biocatalysis and Biotransformations, (J. Whittall, P. Sutton, Eds.); Wiley-VCH: Weinheim, 2009.
  18. P. Bojarová, V. Křen: Azido leaving group in enzymatic synthesis – small and efficient. In Carbohydrate Chemistry: Chemical and Biological Approaches 36 (A. Pilar Rauter, T. K. Lindhorst, Eds.); RSC Publishing: Cambridge, UK, 2010.
  19. K. Bezouška, R. Šnajdrová, K. Křenek, M. Vančurová, A. Kádek, D. Adámek, P. Lhoták, D. Kavan, K. Hofbauerová, P. Man, P. Bojarová, V. Křen: Carboxylated calixarenes bind strongly to CD69 and protect CD69+ killer cells from suicidal cell death induced by tumor cell surface ligands. Bioorg. Med. Chem., 2010, 18.1434–1440.
  20. D. Kavan, M. Kubíčková, J. Bílý, O. Vaněk, K. Hofbauerová, H. Mrázek, D. Rozbeský, P. Bojarová, V. Křen, L. Žídek, V. Sklenář, K. Bezouška: Cooperation between Subunits Is Essential for High-Affinity Binding of N-Acetyl-D-hexosamines to Dimeric Soluble and Dimeric Cellular Forms of Human CD69. Biochemistry 2010, 49, 4060–4067.
  21. K. Slámová, R. Gažák, P. Bojarová, N. Kulik, R. Ettrich, H. Pelantová, P. Sedmera, V. Křen: 4-Deoxy-substrates for β-N-acetylhexosami­nidases: How to make use of their loose specificity. Glycobiology 2010, 20, 1002–1009.
  22. Kristýna Slámová, P. Bojarová, L. Petrásková, V. Křen: -N-Acetylhexosami­nidase: What's in a name…? Biotechnol. Adv. 2010, 28, 682–693.
  23. P. Bojarová, V. Křen: Glycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short. Chimia 2011, 65, 65–70.
  24. H. Ryšlavá, A. Kalendová, V. Doubnerová, P. Skočdopol, V. Kumar, Z. Kukačka, P. Pompach, O. Vaněk, K. Slámová, P. Bojarová, N. Kulik, R. Ettrich, V. Křen, K. Bezouška: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosami­nidase. FEBS J. 2011, 278, 2469–2484.
  25. P. Bojarová, K. Slámová, K. Křenek, R. Gažák, N. Kulik, R. Ettrich, H. Pelantová, M. Kuzma, S. Riva, D. Adámek, K. Bezouška, V. Křen: Charged Hexosaminides as New Substrates for b-N-Acetylhexosami­nidase-Catalyzed Synthesis of Immunomodulatory Disaccharides. Adv. Synth. Catal. 2011, 353, 2409–2420.
  26. C. Rech, R. R. Rosencrantz, K. Křenek, H. Pelantová, P. Bojarová, C. E. Römer, F. G. Hanisch, V. Křen, L. Elling: Combinatorial One-Pot Synthesis of Poly-N-acetyllactosamine Oligosaccharides with Leloir-Glycosyltransfe­rases. Adv. Synth. Catal. 2011, 353, 2492–2500.
  27. A. Drozdová, P. Bojarová, K. Křenek, L. Weignerová, B. Henssen, L. Elling, H. Christensen, H. H. Jensen, H. Pelantová, M. Kuzma, K. Bezouška, M. Krupová, D. Adámek, K. Slámová, V. Křen: Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors. Carbohydr. Res. 2011, 346, 1599–1609.
  28. K. Slámová, P. Bojarová, D. Gerstorferová, B. Fliedrová, J. Hofmeisterová, M. Fiala, P. Pompach, V. Křen: Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosami­nidase in Pichia pastoris. Prot. Express. Purif. 2012, 82, 212–217.
  29. T. Desmet, W. Soetaert, P. Bojarová, V. Křen, L. Dijkhuizen, V. Eastwick-Field, A. Schiller: Enzymatic glycosylation of small molecules: Challenging substrates require tailored catalysts. Chem. Eur. J. 2012, 18, 10786–10801.
  30. H. Mrázek, L. Weignerová, P. Bojarová, P. Novák, O. Vaněk, K. Bezouška: Carbohydrate synthesis and biosynthesis technologies for cracking of the glycan code: Recent advances. Biotechnol. Adv., 2012, in press.
  31. P. Bojarová, R. R. Rosencrantz, L. Elling, V. Křen: Enzymatic glycosylation of multivalent scaffolds. Chem. Soc. Rev. 2013, 42, 4774–4797
  32. J. Krejzová, P. Šimon, Ľ. Kalachová, N. Kulik, P. Bojarová, P. Marhol, H. Pelantová, J. Cvačka, R. Ettrich, K. Slámová, V. Křen: Inhibition of GlcNAc-processing glycosidases by C-6-azido-NAG-thiazoline and its derivatives. Molecules, 2014, 19, 3471–3488.
  33. A. Šimonová, C. E. Kupper, S. Böcker, A. Müller, K. Hofbauerová, H. Pelantová, L. Elling, V. Křen, P. Bojarová: Chemo-enzymatic synthesis of LacdiNAc dimers of varying length as novel galectin ligands. J. Mol. Catal. B: Enzym., 2014, 101, 47–55.

Last update: 22/10/2015

Staff of The Laboratory

Institute of Microbiology - Czech Academy of Sciences