Prof. Vladimír Křen, PhD, DSc, FRSC

Position:

Senior Research Fellow, Head of Laboratory

Email:

kren (at) biomed.cas.cz

Phone:

(+420) 296 442 510

Fax:

(+420) 296 442 509

Address:

Laboratory of Biotransformation,
Institute of Microbiology,
Academy of Sciences of Czech Republic

Videňská 1083
Praha 4
142 20

Czech Republic

Education

• Institute of Chemical Technology in Prague (fermentation chemistry and biotechnology) – MSc (Hons) (1981)
• Czechoslovak Academy of Sciences, Institute of Microbiology, Prague – PhD in biology/micro­biology (1988). Title of the thesis: .
• Associated Professor in bioorganic chemistry – Palacky University, Olomouc, Institute of Medical Chemistry and Biochemistry (1998). Habilitation thesis:
• Full Professor in Medicinal chemistry, Palacky University, Olomouc, 2005.

Research Orientation

• Ergot alkaloids, chemical and enzymatic modifications
• Immobilized microbial cells, their use in production and biotransformation of natural products
• Biotransformation of natural products by enzymes and microorganisms
• Preparation of glycosidases of microbial origin and their use for glycosylation of natural compounds
• Glycoconjugates, multivalent compounds, immunological applications
• Antioxidants and chemoprotectants based on silybin

Coordinated projects

• Industrial grant – 1994, 1995
• Grant of Czech Academy of Sciences – 1994 – 1996
• EU Grant PECO Preparation of ergot alkaloid O- and N- glycosides with potential antiviral activities.
• EU Grant PECO Glycosylation of flavonoids with potential hepatoprotective activities.
• Volkswagen foundation res. grant. (together with Prof. J. Thiem, Univ. Hamburg) – Chemoenzymatic synthesis of immunomodulatory saccharides
• DAAD grant (together with Dr. T.K. Lindhorst, Univ. Hamburg) – Dendrimeric carbohydrates
• Grant of Czech National Grant Agency – 6 grants up to now
• Grant of Czech Ministry of Industry – 3 grants up to now
• Grant of Czech Academy of Sciences – 4 grants up to now
• Grant of British Royal Society of Chemistry (3×)
• National Center for Biotransformation and Biocatalysis (coordinator)

Membership in Scientific Societies

• Czech Society of Biochemistry
• Czech Society of Chemistry
• Czechoslovak Society of Microbiology
• British Royal Society of Chemistry (FRSC)
• American Chemical Society

Membership in Editorial Boards

• Journal of Carbohydrate Chemistry
• Biotransformation and Biocatalysis

Scientific-organizatory activities

• Head of laboratory (15 research workers & PhD students) – date
• Head of department – 2001–2003
• Vice-chairman of Scientific board of the Institute of Microbiology, Academy of Sciences 1996–2001.
• Member of Scientific board of the Inst. of Organic Chemistry and Biochemistry, Acad. Sci. 1992–2001
• Chairman of Chemistry section of the Grant Agency of the Cz. Acad. Sci. 1995–98
• Editorial board of Journal of Carbohydrate Chemistry – member
• Representative of the Czech Republic in EUROCARB (European Carbohydrate Organization)
• Representative of the Czech Republic in ICO (International Carbohydrate Organization)

Consultation Activity

• 1987 – date Galena Pharmaceuticals (Opava, Czech Rep.) – fermentative preparation and biotransformation of ergot alkaloids. Fermentative production of fungal metabolites, biotransformations of bio-active substances, flavonoid chemistry.

Expert Activity for the Czech Government

• Ministry of Industry and Commerce – Member of ministerial advisory board for industrial grants
• Ministry of Education – Member of the ministerial advisory board for young scientists returning from foreign stays
• Ministry of Education – Member of the ministerial advisory board for institutional grants

Expert Activity for EU

• Evaluation of grant applications of 5th and 6th FW – Food quality and microbiology
• Evaluation of applications for Marie Curie training centers and Centers of Excellence CERC
• Preparation of the position materials for EU (SusChem)

Participation in COST activities

• MC member of Cost Chemistry D25, D28 and D34
• Coordinator of WG D25–0001–02
• Participant in WG D13–0001–98 and WG D13–0015–01, WG D28–0007–03, WG D34–0001–05
• Organizer of Workshop of COST D25, June 28, 2003 Olomouc

Awards

• Distinguished Young Scientist Achievement Medal – Czechoslovak Academy of Sciences (1988)
• Alexander von Humboldt Research Fellowship (1993)
• Matsumae International Foundation Research Award, Japan (1995–6)
• Invited lecture – Gordon conference Biocatalysis, Boston (1996)
• Czech Academy of Sciences Award for Excellent project results (2003)

Hobbies

• Biking
• Swimming
• Scuba-diving

Publications

• Ergot alkaloids & Claviceps
• Silybin/silymarin & other natural products
• Glycosciences
• Biotransforma­tions

Ergot alkaloids & Claviceps

V. Křen, E. Eich, H. H. Pertz: Interaction of the Dopaminergic Drugs Pergolide and Terguride, and their Dimers with 5-HT_2A Receptors of Rat Tail Artery. Physiol. Res. 53 (1) 35 – 43 (2004).
• Köhler, Carola Kraft, Kristina Jenett-Siems, Heinz H. Pertz, V. Křen, A. Fišerová, M. Kuzma, J. Ulrichová, U. Bienzle, E. Eich: In Vitro Antiplasmodial Activities of Semisynthetic N,N’-Spacer-Linked Oligomeric Ergolines. Bioorganic & Medicinal Chemistry 12, 817–824 (2004).
• V. Křen, A. Fišerová, L. Weignerová, I. Stibor, P. Halada, V. Přikrylová, P. Sedmera, M. Pospíšil: Clusterred ergot alkaloids modulate cell-mediated cytotoxity. Bioorganic Medicinal Chemistry 10 (2), 415–424 (2002).
• V. Křen, L. Weignerová, M. Kuzma, A. Jegorov, P. Sedmera: Semisynthetic dimers of antipakinsonic ergot alkaloids. Heterocycles 55 (6) 1045–1056 (2001).
• V. Křen, P. Halada, P. Sedmera: Agroclavine glycosylation. Coll. Czechoslov. Chem. Commun. 64 (1),114–118 (1999).
• V. Křen, L. Cvak (eds.): Ergot – Genus Claviceps, Medicinal & Aromatic Plants – Industrial Profiles (Series editor R. Hardman), Harwood Academic Publ., Ltd., Amsterodam, London (1999).
V. Křen: Biotransformations of ergot alkaloids. Ergot – Genus Claviceps, Medicinal & Aromatic Plants – Industrial Profiles (V. Křen, L. Cvak eds.), Harwood Academic Publ., Ltd., Amsterodam, London (1999)..
• V. Křen: Physiological regulation of ergot alkaloid production and special cultivation techniques. Ergot – Genus Claviceps, Medicinal & Aromatic Plants – Industrial Profiles (V. Křen, L. Cvak eds.), Harwood Academic Publ., Ltd., Amsterodam, London (1999)..
• V. Křen: Enzymatic and chemical glycosylations of ergot alkaloids and biological aspects of new compounds. Topics in Current Chemistry 186, 45 – 65 (1997). ISBN 3–540–62033–8
• V. Křen, A. Pískala, P. Sedmera, V. Havlíček, V. Přikrylová, M. Witvrouw, E. De Clercq : Synthesis and antiviral evaluation of N-β-D-ribosides of ergot alkaloids. Nucleosides Nucleotides 16 (1&2), 97–106 (1997).
• V. Křen, P. Olšovský, V. Havlíček, P. Sedmera, M. Witvrouw, E. De Clercq: N-Deoxyribosides of ergot alkaloids: Synthesis and biological activity. Tetrahedron 53(12), 4503–4510 (1997).
• Fišerová, H. Kovářů, Z. Hajduová, V. Mareš, V. Křen, M. Flieger, M. Pospíšil: Neuroimmunomo­dulation of natural killer (NK) cells by ergot alkaloid derivatives. Physiol. Res. 46 (2), 119–125 (1997).
• V. Křen, P. Sedmera: N-1-TMS Derivatives of Ergot alkaloids. Coll. Czech. Chem. Commun. 61, 1248 – 1253 (1996).
• V. Křen, J. Němeček, V. Přikrylová: Assignment of nitrogen stereochemistry of agroclavine and elymoclavine 6-N-oxides. Coll. Czech. Chem. Commun. 60 (12), 2165 – 2169 (1995).
• V. Křen, P. Harazim, Z. Malinka: Claviceps purpurea (Ergot): Cultivation and ergot alkaloid bioproduction. in Medicinal and aromatic plants VII (ed. Y.P.S. Bajaj), Biotechnology in Agriculture and Forestry, Vol. 28, Springer Verlag, Heidelberg 139 – 156 (1994) ISBN 3–540–57446–8
• V. Křen: Immobilization and coimmobilization of filamentous fungi Claviceps purpurea, C. fusiformis and C. paspali. in Industrial Biotechnology, pp. 335 – 342 (eds. V.S. Malik, P. Sridhar ) Oxford & IBH Publishing Co., Ltd., New Delhi (1992)
V. Křen, S. Pažoutová, T. Řezanka, I. Víden, E. Amler, P. Sajdl: Regulation of lipid and ergot alkaloid biosynthesis in Claviceps purpurea by chlorophenoxy acids. Biochem. Physiol. Pflanzen 186, 99 – 108 (1990).
• S. Pažoutová, V. Křen, P. Sajdl: The inhibition of clavine biosynthesis by 5-F-Trp; an useful tool for the study of regulatory and biosynthesis relationships in Claviceps. Appl.Microbiol. Biotechnol. 33, 330 – 334 (1990).
• V. Křen: Complex exploitation of Claviceps immobilized cells. in :Physiology of immobilized cells, Proc. Int. Symp., Wageningen, 10. – 13. 12. 1989 (J.de Bont, J. Visser, B. Mattiasson, J. Tramper eds.), pp.551 – 556 Elsevier, Amsterodam, 1990.
• V. Křen, M. Flieger, P. Sajdl (1990) Glycosylation of ergot alkaloids by free and immobilized Claviceps purpurea cells. Appl. Microbiol. Biotechnol., 32, 645–650.
• M. Flieger, N.F. Zelenkova, P. Sedmera, V. Křen, J. Novák, V. Rylko, P. Sajdl, Z. Řeháček: Ergot alkaloid glycosides from saprophytic cultures of Claviceps I. Elymoclavine fructosides. J. Nat. Prod. (Lloydia) 52, 506 – 510 (1989).
• M. Flieger, V. Křen, N.F. Zelenkova, P. Sedmera, J. Novák, P. Sajdl: Ergot alkaloids glycosides from saprophytic cultures of Claviceps II. Chanoclavine fructosides. J. Nat. Prod. (Lloydia) 53, (1) 171 – 175 (1989).
• S. Pazoutova, V. Křen, T. Řezanka, E. Amler, M. Flieger, V. Rylko, P. Sajdl: Effect of triadimefon on lipids, sterols, and membrane fluidity in submerged cultures of Claviceps purpurea. Pestic. Biochem. Physiol. 34, 211 – 217 (1989).
• V. Křen, J. Břemek, M. Flieger, J. Kozová, Z. Malinka, Z. Řeháček: Bioconversion of agroclavine by free and immobilized Claviceps fusiformis cells. Enzyme Microbiol. Technol. 11, 685 –691 (1989).
• M. Flieger, J. Votruba, V. Křen, S. Pažoutová, V. Rylko, P. Sajdl, Z. Řeháček: Physiological control and process kinetics of clavine alkaloid production by Claviceps purpurea . Appl. Microbiol. Biotechnol. 29, 181 – 185 (1988).
• S. Pažoutová, V. Křen, T. Řezanka , P. Sajdl: Effect of clomiphene on fatty acids, sterols and membrane fluidity in clavine producing Claviceps purpurea strains. Biochem. Biophys. Res. Commun. 152, 190 – 196 (1988).
• V. Křen, J. Kozová, J. Ludvik, O. Kofroňová, Z. Řeháček: Physiological activity of immobilized Claviceps cells producing clavine alkaloids. Česká mykologie 42, 52 – 55 (1988).
• T. Rezanka, V. Křen, P. Sajdl, Z. Řeháček: Identification of an unusual 4,5-dimethyloctanoic acid in a submerged cultures of Claviceps purpurea . J. Nat. Prod. (Lloydia) 50, 335 (1987).
• V. Křen, J. Ludvik, O. Kofroňová, J. Kozová, Z. Řeháček: Physiological activity of immobilized cells of Claviceps fusiformis during long-term semicontinuous cultivation. Appl. Microbiol. Biotechnol. 26, 219 – 226 (1987).
• S. Pažoutová, M. Flieger, V. Rylko, V. Křen, P. Sajdl: Effect of cultivation temperature, Clomiphene, and Nystatin on the oxidation and cyclization of chanoclavine in submerged cultures of the mutant strain Claviceps purpurea 59. Curr. Microbiol. 15, 97 – 101 (1987).
• V. Křen, P. Mehta, V. Rylko, M. Flieger, J. Kozová, Z. Řeháček: Substrate regulation of elymoclavine formation by some saccharides. Zentralblatt fur Mikrobiol. 142, 71 – 85 (1987).
• V. Křen, S. Pažoutová, V. Rylko, Z. Řeháček: Saprophytic production of clavine alkaloids and activity of 3-hydroxy-3-methylglutaryl coenzyme-A reductase. Folia Microbiol. 31, 282 – 287, (1986).
• V. Křen, T. Řezanka, P. Sajdl, Z. Řeháček: Identification of sterols in submerged cultures of different Claviceps species. Biochem. Physiol. Pflanzen, 181, 505 – 510 (1986).
• V. Křen, S. Chomatová, J. Břemek, P. Pilát, Z. Řeháček: Effect of some broad-spectrum antibiotic on the high-production strain Claviceps fusiformis W1. Biotechnol. Lett. 8, 327 – 332 (1986).
• V. Křen, S. Pažoutová, P.Sedmera, V. Rylko, Z. Řeháček: High-production mutant Claviceps purpurea 59 accumulating secoclavines. FEMS Microbiol. Lett. 37, 31 – 34 (1986).
• V. Křen, T. Řezanka , Z. Řeháček: Occurrence of ricinoleic acid in submerged cultures of various Claviceps species. Experientia 41, 1476 – 1477 (1985).
• V. Křen, T. Řezanka, P. Sajdl, Z. Řeháček: Identification of fatty acids in submerged cultures of Claviceps species. FEMS Microbiol. Letters 30, 359 – 363 (1985).
• V. Křen, Z. Řeháček: Feedforward regulation of phosphofructokinase in a submerged culture of Claviceps purpurea producing clavine alkaloids. Speculat. Sci. Technol. 7, 223 – 226 (1984).
• V. Křen, S. Pažoutová, V. Rylko, P. Sajdl, M. Wurst, Z. Řeháček: Extracellular metabolism of sucrose in a submerged culture of Claviceps purpurea in relation to formation of monosaccharides and clavine alkaloids. Appl. Environ. Microbiol. 48, 826 – 829 (1984).

Silybin/silymarin & other natural products

K. Valentová, D. Buckiová, V. Křen, J. Pěknicová, J. Ulrichová, V. Šimánek: The in vitro biological activity of Lepidium meyenii extracts. Cell Biol. Toxicol. 22, 91–99 (2006).
• P. Džubák, M. Hajdúch, R. Gažák, A. Svobodová, J. Psotová, D. Walterová, P. Sedmera, V. Křen: New derivatives of silybin and 2,3-dehydrosilybin and their cytotoxic and P-glycoprotein modulatory activity. Bioorg. Med. Chem. 14 (11) 3793 – 3810 (2006)
• M. Plíšková, J. Vondráček, V. Křen, R. Gažák, P. Sedmera, D. Walterová, J. Psotová, V. Šimánek, M. Machala: Effects of Silymarin Flavonolignans and Synthetic Silybin Derivatives on Estrogen and Aryl Hydrocarbon Receptor Activation. Toxicology215 (1–2), 80–89 (2005)
• V. Křen, D. Walterová: Silybin and silymarin – New efects and applications. Biomed. Papers 149 (1), 29–41 (2005)
• R. Gažák, A. Svobodová, J. Psotová, P. Sedmera, V. Přikrylová, D. Walterová and V. Křen Oxidised Derivatives of Silybin and their Antiradical and Antioxidant Activity. Bioorganic & Medicinal Chemistry 12, 5677 – 5687 (2004)
• N. Škottová, J. Ulrichová, V. Křen, J. Urbaníková, V. Šimánek: Flavonoidy silymarinu a polynenasycené mastné kyseliny rybízového oleje jako nutraceutikum v prevenci chronických onemocnění. Praktický lékař 83 (8), 444–450 (2003).
• P. Kosina, V. Křen, R. Gebhardt, F. Grambal, J. Ulrichová, D. Walterová: Antioxidant properties of silybin glycosides. Phytother. Res. 16, S33-S39 (2002).
• J. Kubisch, P. Sedmera, P. Halada, R. Gažák, N. Škottová, V. Šimánek and V. Křen: Chemoenzymatic preparation of oligoglycosides of silybin, the flavonolignan from Silybum marianum. Heterocycles, 53 (2), 901–915 (2001).
• V. Šimánek, D. Walterová, J. Vičar, J. Urbaníková, V. Křen, M. Modrianský, N. Škottová, J. Ulrichová: „Silymarin“, extract from the milk thistle (Silybum marianum) – medicine or a nutraceutics? (in Czech), Česká a Slovenská Farmacie 50 (2), 66–69 (2001)
• V. Šimánek, V. Křen, J. Ulrichová, J. Vičar, L. Cvak: Silymarin: What is in the name..? Hepatology 32 (2), 442–443 (2000).
• V. Křen, J. Ulrichová, P. Kosina, D. Stevenson, P. Sedmera, V. Přikrylová, P. Halada, V. Šimánek: Chemoenzymatic preparation of silybine β-glucuronide and their biological evaluation. Drug Metabolism and Disposition, 28 (12), 1513–1517 (2000 ).
• L. Martínková, P. Patáková-Jůzlová, V. Křen, Z. Kučerová, V. Havlíček, P. Olšovský, O. Hovorka, B. Říhová, D. Veselý, D. Veselá, J. Ulrichová, V. Přikrylová: Biological activities of oligoketide pigments of Monascus purpureus. Food Aditives and Contaminants 16 (1), 15–24 (1999).
• V. Křen, A. Minghetti, P. Sedmera, V. Havlíček, V. Přikrylová, N. Crespi-Perellino: Glucosylation of silybin by plant cell cultures of Papaver somniferum var. setigerum. Phytochemistry 47, 217–220 (1998).
• V. Křen, P. Sedmera, J. Kubisch, P. Halada, V. Přikrylová, A. Jegorov, L. Cvak, R. Gebhardt, J. Ulrichová, V. Šimánek: Glycosylation of silybin. J. Chem. Soc., Perkin 1 1997 (17), 2467–2474 (1997).
• P. Jůzlová, T. Řezanka, L. Martínková, V. Křen: Identification of long-chain fatty acids from Monascus, the producer of red pigments. Phytochemistry, 43 (1), 151 – 153 (1996).
• P. Jůzlová, L. Martínková, V. Křen: Secondary metabolites of the fungus Monascus. J. Ind. Microbiol.16, 163 – 170 (1996)
• V. Křen, T. Řezanka: Sterol and fatty acids in Peziza muralis. Can. J. Microbiol. 42, 1176 1178 (1996).
• R. Kysilka, V. Křen: Determination of Lovastatin (Mevinolin) and mevinolinic acid in fermentation liquids. J. Chromatogr. 630, 415 – 417 (1993).

Biotransformations

O. Kaplan, V. Vejvoda, O. Plíhal, P. Pompach, D. Kavan, P. Fialová, K. Bezouška, M. Macková, M. Cantarella, V. Jirků, V. Křen, L. Martínková: Purification and characterization of a nitrilase from Aspergillus niger K10. Appl. Microbiol. Biotechnol. in press 2006
• R. Šnajdrová, V. Kristová-Mylerová, D. Crestia, K. Nicolaou, M. Kuzma, E. Gallienne, G. Lemaire, J. Bolte, K. Bezouška, V. Křen, L. Martínková. Nitrile biotransformation by Aspergillus niger. J. Mol. Catal./B Enzymatic 29, 227–232 (2004)
• L. Martínková, V. Křen: Nitrile- and amide-converting microbial enzymes: stereo-, regio- and chemoselectivity. Biocatalysis and Biotransformation 20 (2), 73–93 (2002)
• L. Martínková, N. Klempier, M. Ovesná, M. Kuzma, P. Sedmera, H. Griengl, V. Křen: Biotransformation of alicyclic hydroxynitriles by Rhodococcus equi A4. Can. J. Chem. 80, 724–727 (2002).
• Přepechalová, L. Martínková, A. Stolz, M. Ovesná, K. Bezouška, J. Kopecký, V. Křen: Purification and characterization of the Enantioselective Nitrile Hydratase from Rhodococcus equi A4. Appl. Microbiol. Biotechnol. 55, 150–156 (2001).
• L. Martínková, N. Klempier, J. Bardakji, A. Kandelbauer, M. Ovesná, T. Podařilová, M. Kuzma, I. Přepechalová, H. Griengl, V. Křen: Biotransformation of 3-substituted methyl (R,S)-4-cyanobutanoates with nitrile-converting biocatalysts. J. Mol. Catal. B: Enzymatic, 14, 95–99 (2001).
• L. Martínková, V. Křen, L. Cvak, M. Ovesná, I. Přepechalová: Hydrolysis of lysergamide to lysergic acid by Rhodococcus equi A4. J. Biotechnol., 84 (1), 63–66 (2000).
• L. Martínková, N. Klempier, I. Přepechalová, V. Přikrylová, M. Ovesná, H. Griengl, V. Křen: Chemoselective biotransformation of nitriles by Rhodococcus equi A4 Biotechnol. Lett. 20 (10) 909–912 (1998).
• V. Křen, L. Kawuloková, P. Sedmera, M. Polášek, T. K. Lindhorst, K.-H. van Pée: Biotransformation of ergot alkaloids by haloperoxidase from Streptomyces aureofaciens: stereoselective acetoxylation and propionoxylation. Liebigs. Ann./Recueil 1997, 2379–2383, (1997).
• S. Borneman, D.H.G. Crout, H. Dalton, V. Křen, M. Lobell, G. Dean, N. Thomson, M.M. Turner: Stereospecific formation of R-aromatic acyloins by Zymomonas mobilis pyruvate decarboxylase. J.Chem.Soc., Perkin Trans. 1 (1996) 425 – 430.
• Svatoš, Z. Huňková, V. Křen, M. Hoskovec, D. Šaman, K. Konečný, I. Valterová, B. Koutek: Stereoselectivity of 3-methyl-1-(phenylsulfonyl)-hexan-2-one reductions by yeast. Tetrahedron: Asymmetry, 7 (5), 1285 – 1294 (1996).
• V. Křen, P. Sedmera, V. Přikrylová, M. Polášek, A. Minghetti, N. Crespi-Perellino, T. Macek: Biotransformation of chanoclavine by Euphorbia calyptrata cell culture. J. Nat. Prod. 59, 481 – 484 (1996).
• V. Křen, P. Sedmera, M. Polášek, A. Minghetti, N. Crespi-Perellino: Dimerisation of lysergene by Euphorbia calyptrata cell cultures. J. Nat. Prod. 59, 609 – 611 (1996).
• T. Macek, M. Macková, J. Očenášková, K. Demnerová, J. Pazlarová, V. Křen: Peroxidase isoenzyme pattern and total activity changes in plant cells cultivated in vitro under abiotic stress. in Plant Peroxidases: Biochemistry and Physiology, Obinger C., Burner U., Ebermann R., Penel C., Greppin H., eds., Univ. of Geneva, Geneva 1996 ISBN2–88164–008–7
• M. Ščigelová, T. Macek, A. Minghetti, M. Macková, P. Sedmera, V. Přikrylová, V. Křen: Biotransformation of ergot alkaloids by plant cell cultures with high peroxidase activity. Biotechnol. Lett. 17 (11), 1213–1218 (1995).
• L. Martínková, P. Olšovský, I. Přepechalová, V. Křen: Biotransformation of aromatic dinitriles using Rhodococcus equi cells. Biotechnol. Lett. 17 (11), 1219–1222 (1995).
• V. Křen, D.H.G. Crout, H. Dalton, D.W. Hutchinson, W. Konig, G. Dean, N. Thompson: Pyruvate decarboxylase: a new enzyme for the production of acyloins by biotransformations. J. Chem. Soc. Chem. Comm. (1993), 341 – 343.
• V. Křen, M. Kochtová, Z. Burianec, M. Rychtera: Continuous cultivation of Candida utilis growing on calcium bisulphite waste liquors. Parameter estimation based upon respiration data. Appl. Microbiol. Biotechnol. 22, 329 – 335 (1985).
• V. Křen, M. Kochtová, Z. Burianec, M. Rychtera: Continuous cultivation of Candida utilis growing on spent sulfite liquor. Parameter estimation based upon respiration data. Sci. Papers Inst. Chem. Technol. (Prague), R 6, 99 – 123 (1983).
• M. Rychtera, V. Křen, V. Grégr: Growth of yeast Candida utilis in crotonaldehyde containing media. European J. Appl. Microbiol. Biotechnol. 13, 39–44 (1981).
• M. Rychtera, V. Křen, V. Grégr: A study of the inhibitory effect of crotonaldehyde on the growth of Candida utilis in semisynthetic ethanol media. Sci. Papers Inst. Chem. Technol. (Prague), E 52, 119–151 (1981).

Glycosciences

Pišvejcová, C. Rossi, L. Hušáková, V. Křen, S. Riva, D. Monti: β-1,4-Galactosyltran­sferase-catalyzed glycosylation of sugar derivatives: new acquisitions J. Mol. Catal: B-Enzymatic. 39, 98–104 (2006)
• P. Simerská, M. Kuzma, D. Monti, S. Riva, M. Macková, V. Křen: Unique transglycosylation potential of extracellular α-D-galactosidase from Talaromyces flavus. J. Mol. Catal./B Enzymatic. 39, 128–134 (2006)
• T. Carmona, P. Fialová, V. Křen, R. Ettrich, L. Martínková, A. J. Moreno-Vargas, C. González, I. Robina: Cyanodeoxy-Glycosyl derivatives as substrates for enzymatic reactions. Eur. J. Org. Chem. 2006, 1876–1885 (2006)
• M. Petrušová, B. Pribulová, M. Vojtech, M. Matulová, M. Poláková, J. N. BeMiller, V. Křen, L. Petruš: Extension of the Nef reaction to C-glycosylnitro­methanes. Carbohydr. Res. 341, 2019 – 2025 (2006)
• L. Petrásková, A. Charvátová, V. Přikrylová, V. Kristová, J. Rauvolfová, L. Martínková, J. Jiménez-Barbero, N. Aboitiz, L. Petruš, V. Křen: Preparative production and separation of 2-acetamido-2-deoxymannopyra­noside-containing saccharides using borate-saturated polyolic exclusion gels. J. Chrom. A in press (2006)
• V. Kristová, L. Martínková, L. Hušáková, M. Kuzma, J. Rauvolfová, D. Kavan, P. Pompach, K. Bezouška and V. Křen: A chemoenzymatic route to mannosamine derivatives bearing different N-acyl groups. J. Biotechnol. 115, 157–166 (2005)
• D. Monti, A. Candido, M. Manuel Cruz Silva, V. Křen, S. Riva, B. Danieli: Biocatalyzed generation of molecular diversity: Selective modification of the saponine asiaticoside. Advanced Synthesis & Catalysis 347 (7–8), 1168–1174 (2005)
• P. Fialová, D.-J. Namdjou, R. Ettrich, V. Přikrylová, J. Rauvolfová, K. Křenek, M. Kuzma, L. Elling, K. Bezouška, V. Křen: Combined application of galactose oxidase and β-N-acetylhexosami­nidase in the synthesis of complex immunoactive N-acetylgalacto­saminides. Advanced Synthesis & Catalysis 347 (7–8), 997–1006 (2005)
• V. Šimánek, V. Křen, J. Ulrichová, J. Gallo: The eficacy of glucosamine and chondroitin sulfate in the treatment of osteoarthritis: Are these saccharides drugs or nutraceuticals? Biomed. Papers 149 (1), 51–56 (2005)
• P. Fialová, A. T. Carmona, I. Robina, R. Ettrich, P. Sedmera, V. Přikrylová, L. Hušáková, V. Křen: Glycosyl azides – Novel substrates for enzymatic transglycosyla­tions. Tetrahedron Letters 46, 8715–8718 (2005)
• J. Rauvolfová, L. Weignerová, M. Kuzma, V. Přikrylová, A. Pišvejcová, M. Macková, V. Křen: Enzymatic synthesis of N-acetylglucosa­minobioses by reverse hydrolysis: Characterisation and application of the library of fungal β-N-acetylhexosami­nidases. J. Mol. Catal./B Enzymatic 29, 259–264 (2004).
• N. Aboitiz, F. Javier Cañada, Lucie Hušáková, Marek Kuzma, Vladimír Křen, Jesús Jiménez-Barbero: Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains. Org. Biomol. Chem. 2, 1987–1994 (2004).
• D. Monti, A. Pišvejcová, V. Křen, M. Lama, S. Riva: Generation of an α-L-rhamnosidase library and its application for selective derhamnosylation of natural products. Biotechnol. Bioeng. 87 (6) 763–771 (2004)
• O. Plíhal, J. Sklenář, J. Kmoníčková, P. Man, P. Pompach, V. Havlíček, V. Křen, K. Bezouška. N-Glycosylated catalytic unit meets O-glycosylated propeptide: complex protein architecture in a fungal hexosaminidase. Biochem. Soc. Trans. 32, P5, 764 – 765 (2004)
• P. Fialová, L. Weignerová, J. Rauvolfová, V. Přikrylová, A. Pišvejcová, R. Ettrich, M. Kuzma, P. Sedmera, V. Křen.: Hydrolytic and transglycosylation reactions of N-acyl modified substrates catalysed by β-N-acetylhexosami­nidases. Tetrahedron 60 (3), 693–701 (2004)
• P. Simerská, A. Pišvejcová, M. Kuzma, V. Křen, S. Nicotra, Sergio Riva: Regioselective Enzymatic Acylation of N-Acetylhexosamines. J. Mol. Catal./B Enzymatic 29, 219–225 (2004)
• J. Rauvolfová, M. Kuzma, L. Weignerová, P. Fialová, V. Přikrylová, A. Pišvejcová, M. Macková, V. Křen: β-N-Acetylhexosami­nidase-catalysed synthesis of non-reducing oligosaccharides. J. Mol. Catal./B Enzymatic 29, 233–239 (2004)
• V. Nieder, M. Kutzer, V. Kren, R.G. Gallego, J.P. Kamerling, J.F.G. Vliegenthart, L. Elling: Screening and Characterization of β-N-Acetylhexosami­nidasesfor the Synthesis of Nucleotide-Activated Disaccharides. Enz. Microb. Technol. 34, 404 – 414 (2004).
• P. Krist, L. Vannucci, K. Sadalapure, A. Patel, K. Bezouška, M. Pospíšil, M. Kuzma, T.K. Lindhorst, L. Petruš, V. Křen. Fluorescent Labelled Thiourea-bridged Glycodendrons. ChemBIOChem 5, 445–452 (2004).
• V. Křen: Enzymatic synthesis of carbohydrate ligands for natural killer cells. The way from ligand optimization towards anticancer glycodrugs. Chemical Probes in Biology (Schneider M.P., ed.) 379–389, Kluwer, Amsterodam 2003.
• P. Krist, M. Kuzma, I.P. Pelyvas, P. Simerská, V. Kren: Synthesis of p-nitrophenyl 2-acetamido-2-deoxy-β-D-mannopyranoside and 2-acetamido-2-deoxy-α-D-mannopyranoside. Coll. Czechoslov. Chem. Commun. 68(4), 801–811 (2003)
• L. Weignerová, P. Vavrušková, A. Pišvejcová, J. Thiem, V. Křen: Fungal β-N-acetylhexosami­nidases with high β-N-acetylgalacto­saminidase activity and their use for syntheis of β-GalNAc-containing oligosaccharides. Carbohydr. Res. 338, 1003–1008 (2003)
• L. Hušáková, E. Herkommerová-Rajnochová, T. Semeňuk, M. Kuzma, J. Rauvolfová, R. Ettrich, K. Bezouška, V. Křen: Enzymatic discrimination of GlcNAc and ManNAc-containing disaccharides using β-N-acetylhexosami­nidase. Advanced Synthesis and Catalysis 345 (6–7), 735–742 (2003)
• P. Simerská, M. Kuzma, A. Pišvejcová, L. Weignerová, M. Macková, S. Riva, V. Křen: Application of selectively acylated glycosides for the α-D-galactosidase-catalysed synthesis of disaccharides. Folia Microbiol. 48 (3), 329 – 338 (2003).
• M. Baráth, M. Petrušová, S. Bystrický, V. Křen, L. Petruš: Synthesis of a Precursor of a Lipid A Mimic. Chem. Pap. 57, 125–130 (2003).
• L. Vannucci, A. Fišerová, K. Sadalapure, T.K. Lindhorst, M. Kuldová, P. Rossmann, O. Horváth, V. Křen, P. Krist, K. Bezouška, M. Luptovcová, F. Mosca, M. Pospíšil: Effects of N-acetyl-glucosamine-coated glycodendrimers as biological modulators in the B16F10 melanoma model in vivo. Int. J. Oncol. 23, 285–296 (2003)
• J. Pavlíček, B. Sopko, R. Ettrich, V. Kopecký, V. Baumruk, P. Novák, K. Havlíčková, M. Vrbacký, L. Martínková, V. Křen, M. Pospíšil, K. Bezouška: Molecular Characterization of Binding of Calcium and Carbohydrates by an Early Activation Antigen of Lymphocytes CD69. Biochemistry 42, 9295–9306 (2003)
• M. Casali, L. Tarantini, S. Riva, Z. Huňková, L. Weignerová, V. Křen: Exploitation of a Library of α-Galactosidases for the Synthesis of Building Blocks for Glyco-polymers. Biotechnol. Bioeng. 77(1):105–110 (2002).
• T. Semeňuk, P. Krist, K. Bezouška, M. Kuzma, P. Halada, V. Křen: Synthesis of chitooligomer-based glycoconjugates and their binding to rat natural killer cell activation receptor NKR-P1. Glycoconjugate J. 18, 817–826 (2001)
• J. Dvořáková, D. Schmidt, Z. Huňková, J. Thiem, V. Křen: Enzymatic rearrangement of chitine hydrolysates with β-N-acetylhexosami­nidase from Aspergillus oryzae. J. Mol Catal. B: Enzymatic 11 (4–6), 225–232 (2001).
• L. Weignerová, Z. Huňková, M. Kuzma, V. Křen: Enzymatic Synthesis of three pNP-α-Galabioses: Application of the Library of Fungal α-Galactosidases. J. Mol Catal. B: Enzymatic 11 (4–6), 219–224 (2001).
• V. Křen: Biological Activity – Glycoside vs. Aglycone. in: Glycoscience: Chemistry and Chemical Biology. Vol. III, pp. 2471–2532 (Fraser-Reid, B., Tatsuta, K., Thiem, J., eds.) Springer, Heidelberg, 2001.
• V. Křen, L. Martínková: Glycosides in medicine: "The role of glycosidic residue in biological activity". Curr. Med. Chem. Rev. 8, 1313–1338 (2001).
• L. Hušáková, S. Riva, M. Casali, S. Nicotra, M. Kuzma, Z. Huňková, V. Křen: Enzymatic glycosylation using 6-O-acylated sugar donors and acceptors: β-N-acetylhexosami­nidase catalysed synthesis of 6-O,N,N’-triacetyl-chitobiose and 6’-O,N,N’-triacetylchito­biose. Carbohydr. Res. 331 (2), 143–148 (2001).
L. Weignerová, J. Spížek, L. Najmanová, V. Křen: Enzymatic glycosylation of linkomycin. Bioscience, Biotechnology, and Biochemistry 65 (8), 1897 – 1899 (2001)
• M. Zarevúcka, M. Vacek, Z. Wimmer, Z. Huňková, V. Křen: Enzymic Synthesis and Hydrolysis of Alkyl-β-D-glucopyranosides. Biotechnol. Lett. 23 (18) 1505–1515 (2001).
P. Krist, E. Herkommerová-Rajnochová, J. Rauvolfová, T. Semeňuk, P. Vavrušková, J. Pavlíček, K. Bezouška, L. Petruš, V. Křen: Towards an optimal oligosaccharide ligand for rat natural killer cell activation receptor NKR-P1. Biochem. Biophys. Res. Commun. 287 (1) 11–20 (2001).
• M. Pospíšil, L. Vannucci, A. Fišerová, K. Krausová, O. Horváth, V. Křen, F. Mosca, K. Sadalapure, T.K. Lindhorst, K. Bezouška. Glycodendrimeric ligands of C-type lectin receptors as therapeutic agents in experimental cancers. Adv. Exp. Biol. Med., 495, 343–348 (2001).
• D. Stevenson, V. Křen, P. Halada, P. Sedmera: Chemoenzymatic preparation of lysergol β-glucuronide. Coll. Czechoslov. Chem. Commun. 65 (1), 117–121 (2000).
• J. Dvořáková, J. Kopecký, V. Havlíček, V. Křen: Formation of inositol phosphates by Aspergillus niger phytase. Folia Microbiol. 45 (2), 128–132 (2000).
• Z. Huňková, A. Kubátová, L. Weignerová, V. Křen: Induction of extracellular glycosidases in filamentous fungi and their potential use in chemotaxonomy. Czech Mycology 51 (1), 71 – 87 (1999).
• L. Weignerová, E. Rajnochová, V. Křen: Enzymová reversní glykosylace. Chem. Listy 93 (12), 781–787 (1999).
• M. Zarevúcka, M. Vacek, J. Kuldová, D. Šaman, Z. Wimmer, L. Weignerová, V. Křen: β-Glycosidases: Tool in the chiral discrimination. Chirality 11, 451–458 (1999).
• L. Weignerová, Y. Suzuki, P. Sedmera, V. Havlíček, R. Marek, V. Křen: Pyridoxine as a Substrate for Screening Synthetic Potential of Glycosidases. Coll. Czechoslov. Chem. Commun. 64 (8),1325–1334 (1999).
• J. Kubisch, L. Weignerová, S. Kötter, T. K. Lindhorst, P. Sedmera, V. Křen: Enzymatic synthesis of p-nitrophenyl β-chitobioside. J. Carbohydr. Chem. 18 (8), 975–984 (1999).
• L. Weignerová, P. Sedmera, Z. Huňková, P. Halada, V. Křen, M. Casali, S. Riva: Enzymatic synthesis of iso-globotriose from partially protected lactose. Tetrahedron Lett. 40 (52), 9297–9299 (1999).
• V. Křen: One pot enzymatic synthesis of sialyl T-antigen. Methods in Biotechnology (C. Bucke, ed.) Humana Press 1999, pp. 165–182.
M. Ščigelová, P. Sedmera, V. Havlíček, V. Přikrylová, V. Křen: Glycosidase-catalyzed synthesis of ergot alkaloid α-glycosides. J. Carb. Chem. 17 (6) 981–986 (1998).
• K. Bezouška, V. Křen, C. Kieburg, T.K. Lindhorst: GlcNAc terminated glycodendrimers form defined precipitates with the soluble dimeric receptor of rat natural killer cells, NKR-P1. FEBS Lett. 426, 243–247 (1998).
• Kieburg, T.K. Lindhorst, V. Křen: Selective enzymatic glycosylation of non-carbohydrate branched polyols. J. Carb. Chem., 17 (8) 1239–1247 (1998).
• T. K. Lindhorst, S. Kötter, J. Kubisch, U. Krallmann-Wenzel, S. Ehlers, V. Křen: Effect of p-Substitution of Aryl α-D-Mannosides on Inhibiting Mannose-Sensitive Adhesion of Escherichia coli – Syntheses and Testing. Eur. J. Org. Chem. 1998, 1669–1674 (1998).
• P. Sedmera, V. Přikrylová, K. Bezouška, E. Rajnochová, J. Thiem, V. Křen: Preparation of ManNAc containing chitooligomers by isomerisation and their binding to NKR-P1 protein. J. Carb. Chem. 17 (9) 1351–1357 (1998).
• V. Křen, E. Rajnochová, Z. Huňková, J. Dvořáková, P. Sedmera: Glycosidase Synthesis of Oligosaccharides: Unusual Nonreducing Sugar GlcNAcb(1–1)bMan Formation by β-N-Acetylhexosami­nidase from Aspergillus oryzae. Tetrahedron Lett. 39 (52) 9777–9780 (1998).
• V. Křen, Z. Huňková, P. Halada, Y. Suzuki: Glycosylation of thiamin by β-N-acetylhexosami­nidases. Biosci. Biotech. Biochem. 62 (12), 2415–2417 (1998).
• V. Křen, J. Dvořáková, U. Gambert, P. Sedmera, V. Havlíček, J. Thiem, K. Bezouška:
  β-Glucosylation of chitooligomers by galactosyltran­sferase. Carbohydr. Res. 305, 517–523 (1998).
• V. Křen, J. Thiem: A simple and non-radioactive method for determination of sialyltransferase activity. Biotechnol. Techniques 11 (5), 323–326 (1997).
• V. Křen, J. Thiem: Glycosylation employing bio-systems: from enzymes to whole cells. Chem. Soc. Rev. 26 (6), 463–474 (1997).
• E. Rajnochová, J. Dvořáková, Z. Huňková, V. Křen: Reversed hydrolysis catalyzed by β-N-acetylhexosami­nidase from Aspergillus oryzae. Biotechnol. Lett. 19(9), 869–872 (1997).
• K. Bezouška, J. Sklenář, J. Dvořáková, V. Havlíček, M. Pospíšil, J. Thiem, V. Křen: NKR-P1 protein, an activating receptor of rat natural killer cells, binds to the chitobiose core of uncompletely glycosylated N-linked glycans, and to linear chitooligomers. Biochem. Biophys. Res. Commun. 238, 149–153 (1997).
• V. Křen, A. Fišerová, C. Augé, P. Sedmera, V. Havlíček, P. Šíma: Ergot alkaloid glycosides with immunomodulatory activities. Bioorg. Med. Chem. 4 (6), 869–876 (1996).
• Z. Huňková, V. Křen, M. Ščigelová, L. Weignerová, O. Scheel, J. Thiem: Induction of β-N-acetylhexosami­nidase in Aspergillus oryzae. Biotechnol. Lett. 18 (6), 725 – 730 (1996).
• V. Křen, J. Thiem: Multienzyme system for a one-pot synthesis of sialyl-T-antigen. Angew. Chem. Int. Ed. Engl. 34 (8) 893–895 (1995)
• Werschkum, W. König, V. Křen, J. Thiem: Substrate structure and incubation parameter dependent selectivities in chiral discrimination of galactopyranosides by β-galactosidase hydrolysis. J.C.S. Perkin Trans. 1 (1995) 2459–2466.
• V. Křen, M. Ščigelová, V. Přikrylová, V. Havlíček, P. Sedmera: Enzymatic preparation of β-N-acetyl hexosaminides of ergot alkaloids. Biocatalysis & Biotransformation 10, 181 – 193 (1994)
• V. Havlíček, M. Flieger, V. Křen, M. Ryska: Fast atom bombardment mass spectrometry of elymoclavine glycosides. Biol. Mass Spectrom. 23, 57 – 60 (1994)
• V. Křen, C. Augé, P. Sedmera, V. Havlíček: -Glucosyl and -galactosyl transfer catalysed by –1,4-galactosyltran­sferase in preparation of glycosylated alkaloids. J.C.S. Perkin Trans. 1 (1994), 2481 – 2484.
• M. Ščigelová, V. Křen, K.G.I. Nilsson: Synthesis of -mannosylated ergot alkaloids employing -mannosidase. Biotechnol. Lett. 16 (7) 683 – 688 (1994).
• V. Křen, A. Svatoš, T. Vaisar, V. Havlíček, P. Sedmera, S. Pažoutová, D. Šaman: Fructosylation of ergot alkaloids by submerged culture Claviceps purpurea inhibited in alkaloid production. J. Chem. Res. (1993), (3) 89
• M. Šůchová, V. Křen, K. Demnerová, T. Macek: Galactose oxidase production by immobilized cells of Dactylium dendroides. Biotechnol. Techniques 6, 309 – 312 (1992)
• V. Křen, P. Sedmera, V. Havlíček, A. Fišerová: Enzymatic galactosylation of ergot alkaloids. Tetrahedron Lett. 33, 7233 – 7236 (1992)
• V. Křen: Fructosylation of ergot alkaloids by yeast invertase. Biotechnol. Lett. 14, 769 – 772 (1992)

Staff of The Laboratory

• Head of the Laboratory of Biotransformation

  • Prof. Vladimír Křen, PhD, DSc, FRSC

• Scientific Staff

  • MSc. Ludmila Martínková, PhD.
  • MSc. Lucie Petrásková-Hušáková, PhD.
  • MSc. Lenka Weignerová, PhD.
  • RNDr. Pavla Fialová-Bojarová, PhD.
  • MSc. Ondřej Kaplan, PhD.
  • MSc. Petr Marhol,
  • RNDr. David Biedermann, PhD.
  • Mgr. Alicja Barbara Veselá,
  • Dr. sc. nat. Michael Kotík, PhD.
  • Ing. Bc. Petr Šimon,
  • Msc. Eva Vavříková, PhD.
  • Msc. Kateřina Fuksová,
  • MSc. Martin Chmátal, PhD.

• PhD Students

  • MSc. Karel Křenek,
  • Ing. Kristýna Slámová,
  • Mgr. Anna Drozdová,
  • Mgr. Alena Petříčková,
  • Mgr. Barbora Fliedrová,
  • Ing. Jana Hofmeisterová,
  • Ing. Daniela Gerstorferová,
  • Mgr. Kateřina Purchartová,

• Technical Staff

  • Pavel Vávra,
  • Drahomíra Dobiášová,
  • Pavla Oubrechtová,

• Undergraduate Students

  • Bc. Anna Rinágelová,
  • Bc. Petra Kolářová,
  • Bc. Michaela Novotná,

• Former Coworkers

  • MSc. Andrea Charvátová-Pišvejcová,
  • RNDr. Věra Přikrylová, PhD.
  • MSc. Radek Gažák, PhD
  • MSc. Jana Klozová,
  • Mgr. Eva Kupčíková,
  • MSc. Pavla Simerská,
  • MSc. Iva Čechová,
  • MSc. Tomáš Filipi,
  • MSc. David Kubáč,
  • MSc. Vojtěch Vejvoda, PhD.
  • Iva Hrdličková,
  • MSc. Pavel Krist, PhD.
  • MSc. Veronika Kristová-Mylerová, PhD.
  • MSc. Jana Rauvolfová, PhD.
  • MSc. Radka Šnajdrová,
  • Mgr. Petra Bašová,
  • MSc. Michal Himl,
  • Jana Horová,
  • MSc. Lucie Živná,
  • Bc. Petra Nováková,
  • MSc. Bronislava Uhnáková, PhD.
  • Zuzana Matějčková,
  • Ing. Veronika Faměrová,